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Image Search Results
Journal: Biology Open
Article Title: Oo-site: A dashboard to visualize gene expression during Drosophila oogenesis suggests meiotic entry is regulated post-transcriptionally
doi: 10.1242/bio.059286
Figure Lengend Snippet: Oo-site allows for visualization of dynamically regulated genes. (A,B) Visualization of expression of RpS19b over germline development from (A) developmentally enriched stages and (B) single-cell seq data indicate that the mRNA level of RpS19b decreases starting in the cysts and is dramatically decreased in early egg chambers. Color indicates relative expression and values indicate the (A) mean TPM±standard error or (B) the normalized expression of RpS19b in each given stage. (C–C″) Confocal images of ovaries with in situ hybridization of RpS19b (green, middle greyscale) and stained for DAPI (blue, right greyscale) demonstrate that the mRNA level of RpS19b decreases starting in the cyst stages and are dramatically lower in early egg chambers consistent with the seq data. (D–D″) Confocal images of ovaries expressing RpS19b::GFP, visualizing (D′) GFP (green, middle greyscale), (D″) Vasa staining (blue, right greyscale), and 1B1 (red) demonstrate that the protein expression of RpS19b::GFP is consistent with its mRNA levels. (E,E′) Quantifications of normalized mean intensity of staining, X-axis represents the distance in microns from the niche, Y-axis represents mean intensity normalized to the maximum mean intensity per germarium of (E) RpS19b mRNA or (E′) RpS19b::GFP. The line represents fit using a loess regression, shaded area represents the standard error of the fit. ( n =5 germaria).
Article Snippet:
Techniques: Expressing, In Situ Hybridization, Staining